The purpose of this experiment is to determine the effect UV light has on the transformation process in E. coli by using a pGAL plasmid DNA sample to identify the performance of the Beta Galactosidase protein. Bacterial transformation is a technique used where a foreign plasmid is introduced into bacteria causing an increased production of the protein for which the plasmid is coded. This new science has the potential to be used in treatments for cancer. Ultraviolet radiation is used in cancer treatments and therapies, thus it is significant to know the effects UV light has on the efficiency of bacterial transformation.
Agar was prepared for eighty plates with X-Gal which, when metabolized by β-Galactosidase produces a blue dye that coats the E. coli cell. Two-thirds of the plates also had ampicillin added to their media. The pGAL plasmid codes for ampicillin resistance and successful transformation should allow for blue colony growth on plates with both X-Gal and ampicillin in their media. Three groups were divided out of 80 plates: Control (X-Gal/noAMP/no pGAL), Experimental 1 (X-GAL/AMP/no pGAL), and Experimental 2 (X-Gal/AMP/pGAL). Within each group half were radiated under UV-C light for 30 seconds each.
The hypothesis, E. coli samples not exposed to UV light will be have a higher rates of pGAL plasmid transformation success indicated by a higher blue colony count, was supported. E. coli plated with pGAL plasmid not exposed to UV light had higher transformation efficiency than E. coli plated with pGAL plasmid exposed to UV light.
Agar was prepared for eighty plates with X-Gal which, when metabolized by β-Galactosidase produces a blue dye that coats the E. coli cell. Two-thirds of the plates also had ampicillin added to their media. The pGAL plasmid codes for ampicillin resistance and successful transformation should allow for blue colony growth on plates with both X-Gal and ampicillin in their media. Three groups were divided out of 80 plates: Control (X-Gal/noAMP/no pGAL), Experimental 1 (X-GAL/AMP/no pGAL), and Experimental 2 (X-Gal/AMP/pGAL). Within each group half were radiated under UV-C light for 30 seconds each.
The hypothesis, E. coli samples not exposed to UV light will be have a higher rates of pGAL plasmid transformation success indicated by a higher blue colony count, was supported. E. coli plated with pGAL plasmid not exposed to UV light had higher transformation efficiency than E. coli plated with pGAL plasmid exposed to UV light.
so the more the blue the better the cure for cancer?
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